Fig. 4. Expression of inflammatory markers in wild-type (WT) and Trpc6^-/- kidneys. Renal mRNA levels of (A) interleukin 1 beta (IL1β), (B) interleukin 6 (IL6) and (C) tumor necrosis factor alpha (TNFα) in sham-operated groups and in UUO-operated groups. Renal mRNA expression data were determined in n=5 each for sham-operated WT and Trpc6^-/- kidneys, n=9 for UUO-operated WT kidneys and n=8 for UUO-operated Trpc6^-/- kidneys. The relative standard curve method was used for relative quantification. All data are means ± SD, ns p>0.05, ***p<0.001, ****p<0.0001. ns, not significant.